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04-18-2012, 06:40 AM
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#1 |
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Junior Member
Location: Israel Join Date: Aug 2011
Posts: 2
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Diluting Library for One Touch
Hi Everyone,
I recently prepared three libraries, and after determining the dilution factors I did emPCR reactions using the one touch on 2 of them. Neither of them seem to have worked based on Qubit results (65% and 115% templated ISPs). I am not sure if I am adding too much of my library or not enough. Does anyone have any experience with this? Thanks Chaim |
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04-23-2012, 01:33 PM
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#2 |
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Senior Member
Location: USA Join Date: Oct 2008
Posts: 158
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you are adding too much if this is before enrichement
Last edited by snetmcom; 04-26-2012 at 04:07 PM. |
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| Tags |
| emulsion pcr, ion torrent, one touch, template prep |
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