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Old 07-27-2012, 12:51 PM   #1
Junior Member
Location: USA

Join Date: Jul 2012
Posts: 1
Default Anyone have enrichment problems?

Hello, I have very difficult time with enrichment. Most libraries need high copies per bead to give >5% enrichment rate. I use 10 copies or more. This is strange to me. I usually use much less than 10 copies per bead. I am afraid this will cause mixed Signals. How come I cant get good values anymore? Anyone have this problem? Thank you for help.
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Old 07-27-2012, 04:59 PM   #2
Location: Ohio

Join Date: Dec 2010
Posts: 95

Yeah, this seems to be happening for a lot of users. I've ad to go all the way to 32 cpb to get 8% with an amplicon pool. No idea why.
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