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05-15-2013, 02:00 AM
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#1 |
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Member
Location: Oxford Join Date: Nov 2010
Posts: 24
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Agilent SureSelect bed files
Hello,
Can anyone tell me what the difference is between these bed files supplied by Agilent for their SureSelect Human All Exon V2 kit: _Regions.bed _Padded.bde _Covered.bed _AllTargets.bed Thanks! |
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07-20-2013, 08:00 AM
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#2 |
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Senior Member
Location: UK Join Date: Jan 2010
Posts: 390
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The padded bed file is the regions that are baited, with an extension of 50 bases to either end. Where this regions meet they are merged into a single region.
These tend to extend into the intronic regions (and generally defines what you can confidently genotype). Regions is the regions the kit is designed to (I think). Covered is the regions that are baited for capture. |
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09-10-2013, 03:53 PM
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#3 |
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Member
Location: Australia Join Date: Mar 2011
Posts: 85
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Very helpful, thanks.
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| Tags |
| bedfile, exome capture |
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