SEQanswers

Go Back   SEQanswers > General



Similar Threads
Thread Thread Starter Forum Replies Last Post
big dye temperature? seqgirl123 General 4 05-09-2013 03:03 AM
alternative for big dye kit ortex RNA Sequencing 0 05-08-2013 01:30 AM
Draw for one free night at the San Diego Hilton! illumina Events / Conferences 1 04-21-2011 12:22 PM
Big Dye Terminator chainTerminator SOLiD 3 07-31-2010 12:27 PM
Big Dye Terminator chainTerminator SOLiD 0 07-29-2010 11:52 AM

Reply
 
Thread Tools
Old 07-23-2013, 08:59 AM   #1
shelleyDNASEQ
Junior Member
 
Location: Portland, Oregon

Join Date: Jul 2013
Posts: 2
Exclamation Big Dye Left out all night

I am just a relief DNA Sequencer and filling in for this week. I'm new to the game...

I left some big dye out last night-about 15 hours at 25C-is it still good?

Suggestions?

Thanks!
shelleyDNASEQ is offline   Reply With Quote
Old 07-24-2013, 02:44 PM   #2
shelleyDNASEQ
Junior Member
 
Location: Portland, Oregon

Join Date: Jul 2013
Posts: 2
Smile my own answer

I tested the stuff out on my pGEM control and it looked fine, so I decided to use it.
shelleyDNASEQ is offline   Reply With Quote
Old 07-29-2013, 07:01 AM   #3
The Spith
Junior Member
 
Location: Oxford, UK

Join Date: Jan 2013
Posts: 2
Default

Divide it into small aliquots so you don't have to constantly freeze-thaw it. That tends to be thing that degrades enzyme the most. It also means that if you leave it out overnight you will at worst only ruin a small amount, and not your whole stock.
The Spith is offline   Reply With Quote
Reply

Tags
big dye, spoiled terminator

Thread Tools

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off




All times are GMT -8. The time now is 02:50 AM.


Powered by vBulletin® Version 3.8.9
Copyright ©2000 - 2021, vBulletin Solutions, Inc.
Single Sign On provided by vBSSO