MiSeq parallel library preparation for targeted mRNA and DNA sequencing?

sganschow · 10-18-2016, 04:03 AM

Hello all,

is there a clever way to (partially) combine library prep for targeted DNA and mRNA sequencing on the MiSeq? Or do they have to be treated completely separate?

I have 30 DNA targets with a size of approx. 300bp and used to follow Illumina's "16S" protocol with overhang primers and Nextera index primers for the 2nd PCR.

Now I'd like to add 20 mRNA targets per sample in the same run and hope that at least some of the steps in the protocols are combinable.

Any advice or thoughts would be greatly appreciated.
Sebastian

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10-18-2016, 04:03 AM	#1
sganschow Junior Member Location: Germany Join Date: Apr 2014 Posts: 4	MiSeq parallel library preparation for targeted mRNA and DNA sequencing? Hello all, is there a clever way to (partially) combine library prep for targeted DNA and mRNA sequencing on the MiSeq? Or do they have to be treated completely separate? I have 30 DNA targets with a size of approx. 300bp and used to follow Illumina's "16S" protocol with overhang primers and Nextera index primers for the 2nd PCR. Now I'd like to add 20 mRNA targets per sample in the same run and hope that at least some of the steps in the protocols are combinable. Any advice or thoughts would be greatly appreciated. Sebastian