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Old 10-27-2010, 10:47 PM   #1
masylichu
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Location: Beijing, China

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Default data set from NCBI SRA

i have downloaded several RNA-seq Data from NCBI SRA, such as SRR019978.fastq.

i want to know whether the raw data has been treated by trimming the adaptor sequence of the RNA-seq. if not, what should i do? and what is the adaptor sequence?

thanks.
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Old 10-28-2010, 01:19 AM   #2
avilella
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I asked this question to the EBI SRA people, and I was told that both NCBI and EBI SRA apply a series of QC procedures before they store the sequences. If you see any noise in the data, like adaptors, please report back to them, because it shouldn't be there.
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Old 10-27-2015, 04:27 PM   #3
VC87
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hi!does anyone knows how to search sra files from Bs seq library fixing metadata features such as organism, tissue, age etc..?thanks
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