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10-27-2010, 10:47 PM
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#1 |
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Member
Location: Beijing, China Join Date: Oct 2010
Posts: 30
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data set from NCBI SRA
i have downloaded several RNA-seq Data from NCBI SRA, such as SRR019978.fastq.
i want to know whether the raw data has been treated by trimming the adaptor sequence of the RNA-seq. if not, what should i do? and what is the adaptor sequence? thanks. |
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10-28-2010, 01:19 AM
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#2 |
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Member
Location: uk Join Date: Mar 2009
Posts: 34
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I asked this question to the EBI SRA people, and I was told that both NCBI and EBI SRA apply a series of QC procedures before they store the sequences. If you see any noise in the data, like adaptors, please report back to them, because it shouldn't be there.
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10-27-2015, 04:27 PM
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#3 |
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Member
Location: Portugal Join Date: Oct 2015
Posts: 18
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hi!does anyone knows how to search sra files from Bs seq library fixing metadata features such as organism, tissue, age etc..?thanks
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