Frozen flow cell

Meliss a · 11-16-2009, 07:00 AM

Has anyone ever used a flow cell that has been frozen. We had a mishap with our refrigerator and my flow cells all froze!
Tech-support advised against using them but I was wondering if anyone has and what the outcome was.

ScottC · 11-16-2009, 01:48 PM

I haven't tried it, but I was told that the flowcells will delaminate if they're frozen, or even put on ice.

Let us know how it goes if you do try!

AndreaStevenesen · 09-16-2014, 03:47 AM

Anyone? @Melissa did you found it? or in elsewhere?

Riva · 09-14-2015, 01:49 AM

Our lab has the experience....>_<. We test run a Miseq flow cell that has been frozen for 3 weeks in its original buffer. We test run the 150 cycles reagent on it and it turns out to be fine in both cluster density and quality.

leibyj · 03-06-2019, 06:42 AM

We had accidentally frozen incorporation buffer and a nano flow cell for two weeks. Thawed both in water at room temperature and successfully sequenced with a 300 cycle kit.

Similar Threads
Thread	Thread Starter	Forum	Replies	Last Post
Loading a flow cell with ssDNA (one strand only)	Yepler	Illumina/Solexa	5	05-23-2011 01:37 PM
Flow cell modifications ?	JYC	Illumina/Solexa	2	04-08-2011 11:52 AM
Method to reuse Illumina flow cell	cybog337	Illumina/Solexa	5	08-24-2010 06:26 PM
Flow cell/channel terminology	Calico	Helicos / Direct Genomics	7	01-26-2010 08:19 AM
sequences on the flow cell	suludana	Illumina/Solexa	0	07-22-2008 08:51 AM

11-16-2009, 07:00 AM	#1
Meliss a Junior Member Location: Ann Arbor, MI Join Date: Nov 2009 Posts: 1	Frozen flow cell Has anyone ever used a flow cell that has been frozen. We had a mishap with our refrigerator and my flow cells all froze! Tech-support advised against using them but I was wondering if anyone has and what the outcome was.

09-16-2014, 03:47 AM	#3
AndreaStevenesen Junior Member Location: London Join Date: Sep 2014 Posts: 1	Anyone? @Melissa did you found it? or in elsewhere? __________________ Visit http://sathyas.com/services/ecommerce

11-16-2009, 01:48 PM	#2
ScottC Senior Member Location: Monash University, Melbourne, Australia. Join Date: Jan 2008 Posts: 246	I haven't tried it, but I was told that the flowcells will delaminate if they're frozen, or even put on ice. Let us know how it goes if you do try!

09-14-2015, 01:49 AM	#4
Riva Junior Member Location: Taipei, Taiwan Join Date: Jul 2013 Posts: 3	Our lab has the experience....>_<. We test run a Miseq flow cell that has been frozen for 3 weeks in its original buffer. We test run the 150 cycles reagent on it and it turns out to be fine in both cluster density and quality.

03-06-2019, 06:42 AM	#5
leibyj Junior Member Location: Philadelphia, USA Join Date: Mar 2019 Posts: 1	We had accidentally frozen incorporation buffer and a nano flow cell for two weeks. Thawed both in water at room temperature and successfully sequenced with a 300 cycle kit.