Hi Seqanswers!
Forgive me if there’s already a post on this (I saw a post comparing Truseq ChIP kit with NEBnext, but nothing on Kapa) but does anyone have any experience using a Kapa hyper or Kapa Whole Genome Library kit for ChIPSeq? I'll be preparing some ChIP libraries for sequencing on the Hiseq and I was thinking I could just start at end repair with my ChIP DNA, make my own primers and adapters, and adjust their concentrations to the lower input. I’ve had excellent luck with Kapa on whole genome long-insert libraries in the past, but have never started with anything less than a 250ng input of gDNA and would like to use about 5-10ng ChIP DNA for this application.
Does anyone have a general preference between Kapa and NEB when it comes to things like reagent quality, workflow, helpfulness of tech support, etc…? I'm also curious as to how the efficiency of the ligation and amplification reactions in their library kits compare.
Thanks! And I’ll make sure to update this with any info I get.
Forgive me if there’s already a post on this (I saw a post comparing Truseq ChIP kit with NEBnext, but nothing on Kapa) but does anyone have any experience using a Kapa hyper or Kapa Whole Genome Library kit for ChIPSeq? I'll be preparing some ChIP libraries for sequencing on the Hiseq and I was thinking I could just start at end repair with my ChIP DNA, make my own primers and adapters, and adjust their concentrations to the lower input. I’ve had excellent luck with Kapa on whole genome long-insert libraries in the past, but have never started with anything less than a 250ng input of gDNA and would like to use about 5-10ng ChIP DNA for this application.
Does anyone have a general preference between Kapa and NEB when it comes to things like reagent quality, workflow, helpfulness of tech support, etc…? I'm also curious as to how the efficiency of the ligation and amplification reactions in their library kits compare.
Thanks! And I’ll make sure to update this with any info I get.
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