Hi everyone,
I'm very new to the world of next generation sequencing, I've been reading a lot about the different types and their advantages and disadvantages.
I want to construct a shotgun metagenomic library to find specific enzymes, but I have read that data from a Miseq is much more difficult to assemble due to the short reads it produces. Is this still true even with the new 250 bp setting?
my options are to use a Roche 454 GS FLX+ or a Miseq
I know 454 has been used to construct metagenomes successfully but I also know using a Miseq is much cheaper, so just looking for a bit of advice about which is best to use.
Thanks in advance!
I'm very new to the world of next generation sequencing, I've been reading a lot about the different types and their advantages and disadvantages.
I want to construct a shotgun metagenomic library to find specific enzymes, but I have read that data from a Miseq is much more difficult to assemble due to the short reads it produces. Is this still true even with the new 250 bp setting?
my options are to use a Roche 454 GS FLX+ or a Miseq
I know 454 has been used to construct metagenomes successfully but I also know using a Miseq is much cheaper, so just looking for a bit of advice about which is best to use.
Thanks in advance!
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