Seqanswers Leaderboard Ad

Collapse

Announcement

Collapse
No announcement yet.
X
 
  • Filter
  • Time
  • Show
Clear All
new posts

  • UltraClean PCR purification kits

    We are having continuing issues with background bacterial sequence contamination in our MiSeq sequencing data that we think we have tracked to the min-elute columns we use to purify and concentrate our dsDNA products prior to library prep. We wanted to try a Mo Bio UltraClean PCR clean-up product - Qiagen now has Mo Bio product but only supplies the PCR clean-up in a 96-well format and we need individual columns.
    Does anyone know of an alternative source or substitution for these "clean" purification columns?
    Thanks!
    gLAH

  • #2
    PCR cleanup should be comparatively "easy" and all the usual manufacturers should have good options.
    Qiagen QIAquick PCR or Zymo DNA clean would be some we have used.

    Comment


    • #3
      How about using bead-based cleanup and just skipping the columns entirely? I have heard of people having trouble with contamination of their PCR-grade water when they have samples with very low levels of template DNA.

      Comment

      Latest Articles

      Collapse

      • seqadmin
        Essential Discoveries and Tools in Epitranscriptomics
        by seqadmin


        The field of epigenetics has traditionally concentrated more on DNA and how changes like methylation and phosphorylation of histones impact gene expression and regulation. However, our increased understanding of RNA modifications and their importance in cellular processes has led to a rise in epitranscriptomics research. “Epitranscriptomics brings together the concepts of epigenetics and gene expression,” explained Adrien Leger, PhD, Principal Research Scientist on Modified Bases...
        Yesterday, 07:01 AM
      • seqadmin
        Current Approaches to Protein Sequencing
        by seqadmin


        Proteins are often described as the workhorses of the cell, and identifying their sequences is key to understanding their role in biological processes and disease. Currently, the most common technique used to determine protein sequences is mass spectrometry. While still a valuable tool, mass spectrometry faces several limitations and requires a highly experienced scientist familiar with the equipment to operate it. Additionally, other proteomic methods, like affinity assays, are constrained...
        04-04-2024, 04:25 PM

      ad_right_rmr

      Collapse

      News

      Collapse

      Topics Statistics Last Post
      Started by seqadmin, 04-11-2024, 12:08 PM
      0 responses
      39 views
      0 likes
      Last Post seqadmin  
      Started by seqadmin, 04-10-2024, 10:19 PM
      0 responses
      41 views
      0 likes
      Last Post seqadmin  
      Started by seqadmin, 04-10-2024, 09:21 AM
      0 responses
      35 views
      0 likes
      Last Post seqadmin  
      Started by seqadmin, 04-04-2024, 09:00 AM
      0 responses
      55 views
      0 likes
      Last Post seqadmin  
      Working...
      X