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Old 08-14-2011, 04:41 PM   #1
Location: US

Join Date: Jul 2010
Posts: 11
Default qPCR reagent recommendation

My facility has recently purchased an Illumina GA IIx. I was wondering for qPCR during sample prep, what kits/reagents people recommend? We have an ABI stepOnePlus instrument.

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Old 08-24-2011, 04:50 PM   #2
Location: USA

Join Date: Jul 2010
Posts: 58

KAPA kit I would recommend
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Old 08-29-2011, 02:55 PM   #3
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Location: Santa Barbara, CA

Join Date: Jan 2011
Posts: 3

If you're looking for a SYBR (or similar)-based master mix, I can tell you that Fisher Scientific has a promotional deal on Promega's GoTaq qPCR mix that is ridiculously cheap. It is sold as 1000 or 200 reactions, but that's based on a 50uL reaction volume, so if you do 25uL reactions, that's the price you'd get for 2000 or 400 reactions.

GoTaq qPCR promo
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