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Old 04-29-2014, 12:33 PM   #1
SLCoon
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Location: Washington DC

Join Date: Apr 2014
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Question ribosomal profiling on a SOLiD 5500?

I'm starting a ribosomal profiling experiment where I want to do the sequencing on a SOLiD 5500xl. I've done some work with Illumina sequencing. Is there a protocol for making the libraries for the SOLiD? I think I understand that I need to use specific DNA/RNA adapters (Adapters A or B), but these do not seem to currently commercially available. Do i need to go ahead and synthesize them myself? Has anyone done this?
Thanks, SLCoon
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