Hi everyone, check out our new sequence capture method, recently published in NBT. We are calling the method oligonucleotide-selective sequencing (OS-Seq). The major innovation involves integrating the capture reaction directly on the Illumina flow cell. This eliminates user error (all capture manipulations are done automatically by the sequencer), high cost and laborious sample preparation steps associated with commercial technologies. Let me know what you guys think/ if you have any questions!
Efficient targeted resequencing of human germline and cancer genomes by oligonucleotide-selective sequencing
With the cost of DNA sequencing falling rapidly, sample preparation is becoming a bottleneck to surveying genetic variation across large populations or performing clinical diagnostics. Myllykangas et al. present an efficient approach for targeted sequencing in which genomic regions of interest are captured and sequenced inside a flow cell using a common oligonucleotide probe.
Jason Buenrostro
Efficient targeted resequencing of human germline and cancer genomes by oligonucleotide-selective sequencing
With the cost of DNA sequencing falling rapidly, sample preparation is becoming a bottleneck to surveying genetic variation across large populations or performing clinical diagnostics. Myllykangas et al. present an efficient approach for targeted sequencing in which genomic regions of interest are captured and sequenced inside a flow cell using a common oligonucleotide probe.
Jason Buenrostro
Comment