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Old 08-08-2019, 02:00 AM   #1
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Default RNA-seq data QC

Regarding trimming of raw RNA-seq datasets, do we need to trim off primer sequences despite FASTQC not indicating these as overrepresented?
Aishah is offline   Reply With Quote
Old 08-08-2019, 04:17 AM   #2
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I assume you are referring to adapter sequences. Strictly speaking you don't need to since most modern aligners will soft clip them.

That said, it can be a good practice to scan and trim your data so you can be sure that there is no extraneous sequence present.
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