Hello,
My lab is working on developing homemade barcoded adapters, as in general the illumina kits are overpriced. No trouble coming up with the barcodes and adapter sequences, but as far as modifications go its hard to tell what is really necessary.
Some labs seem to use HPLC, while elsewhere desalting has been listed as being alright. This is a big one, as it adds a lot to the cost if you order many adapters. Any thoughts?
We were also planning on phosphorylating them ourselves, which should be reliable. Has any body else tried this? What about any of the other modifications?
For our nonbarcoded libraries we already use homemade adapters and primers and don't bother with most of the modifications and our results have been fine, but the investment in ordering all of these adapters is a bit higher I'd like to know others experiences.
Thanks!
My lab is working on developing homemade barcoded adapters, as in general the illumina kits are overpriced. No trouble coming up with the barcodes and adapter sequences, but as far as modifications go its hard to tell what is really necessary.
Some labs seem to use HPLC, while elsewhere desalting has been listed as being alright. This is a big one, as it adds a lot to the cost if you order many adapters. Any thoughts?
We were also planning on phosphorylating them ourselves, which should be reliable. Has any body else tried this? What about any of the other modifications?
For our nonbarcoded libraries we already use homemade adapters and primers and don't bother with most of the modifications and our results have been fine, but the investment in ordering all of these adapters is a bit higher I'd like to know others experiences.
Thanks!
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