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Old 02-25-2013, 10:25 AM   #1
Location: Boston, MA

Join Date: Apr 2011
Posts: 42
Default Sequencing 101 - An Introduction to Next-Gen Sequencing Technology and Applications

In less than five years, next-generation sequencing has gone from radical to routine. Naturally, in a field moving so fast, newbies can feel overwhelmed. The Sequencing 101 short course at X-Gen Congress ( is designed to provide definitions of terminology, types of platforms and workflow strategies, as well as current applications and future directions. Learn about the genomic data revolution from data generation to management to analysis and interpretation. This course is perfect for those needing an introduction to next-generation sequencing technology and applications.

When: March 19, 5:30 - 8:30pm
Where: Hilton San Diego Resort, San Diego, California

Course Outline:

  • Human Genetics and Genomics
  • Conventional sequencing vs. NGS
  • Big data and big biology
NGS Technology
  • Key NGS technology: brief history and technology basics
  • NGS applications: de novo sequencing, resequencing, RNA-seq, reduced genome (exome, ChIP-seq, microRNA etc), metagenome, genotyping by sequencing, personalized genomics etc.
  • Data analysis 101: assembly and alignment, sequence variation detection, database and IT related consideration
Experimental Design
  • Biological question
  • Decision tree
  • Sample prep
  • Data analysis (statistical aspect)

Future Trends of NGS and Genomics
  • Emerging technology
  • Single cell genomics, synthetic genomics, system biology etc.
  • Omics integration
  • Cloud computing

Instructor: Ryan Kim, Ph.D. Director, UC Davis Genome Center, DNA Technologies and Expression Analysis Cores, University of California, Davis

For more details, visit
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