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Old 03-13-2014, 02:27 PM   #1
luc
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Default single buffer library prep - A-tailing enzyme?

Hi,
recently several vendors are offering library prep kits which carry out end-repair and A-tailing without buffer change (simply using a two temperature incubation). The kits do not even require a buffer change/cleanup for the ligation. Please see the links below.

Would you have any idea which enzymes are used in these kits for A-tailing?

https://www.neb.com/products/e7370-n...ina#tabselect2
http://www.thermoscientificbio.com/n...-kits/#claseek
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Old 03-13-2014, 04:36 PM   #2
luc
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Apparently simply Taq polymerase can be used for A-tailing at very low concentrations:

Neiman et al 2012:
http://www.plosone.org/article/info%...l.pone.0048616

The published protocol carries out the ligation at 16 degrees overnight, though.
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