SEQanswers

Go Back   SEQanswers > Bioinformatics > Bioinformatics



Similar Threads
Thread Thread Starter Forum Replies Last Post
cutadapt: A tool that removes adapter sequences mmartin Bioinformatics 132 05-19-2016 07:09 AM
PE rmdup removes only one set of duplicates? waalkes Bioinformatics 0 09-18-2012 08:44 AM
vcfutils.pl varFilter removes INDELs even with default override hansdd Genomic Resequencing 0 08-12-2012 08:24 PM
solid_denovo_postprocessor.pl removes velvet contig sequence. KevinLam Bioinformatics 0 02-09-2010 05:04 PM
what's clipping point? jordi 454 Pyrosequencing 1 05-26-2009 01:47 AM

Reply
 
Thread Tools
Old 06-16-2014, 07:41 AM   #1
sdmoore
Member
 
Location: Florida

Join Date: Jun 2014
Posts: 11
Default re-clipping the same library removes even more?

I am working with Illumina libraries on Galaxy.org.

I have used clip to remove Illumina adapters from the data set, it removed "too shorts, adapter-only, and the N-reads", (it removed >90,000). Strangely, I found if I re-run clip, it pulls even more out (>10,000, mostly "too-shorts and adapter only). Why would re-running the same search find more? I assume that maybe the original processing crated some new targets in the output, but that seems like a heck of a lot.
sdmoore is offline   Reply With Quote
Reply

Thread Tools

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off




All times are GMT -8. The time now is 01:02 PM.


Powered by vBulletin® Version 3.8.9
Copyright ©2000 - 2020, vBulletin Solutions, Inc.
Single Sign On provided by vBSSO