SEQanswers

Go Back   SEQanswers > Sequencing Technologies/Companies > Ion Torrent



Similar Threads
Thread Thread Starter Forum Replies Last Post
Ion Torrent $1000 Genome!? Benchtop Ion Proton Sequencer aeonsim Ion Torrent 88 10-28-2012 05:50 AM
Ion Torrent Services? RealMD Ion Torrent 14 11-23-2011 02:00 PM
ion torrent herrroaa Introductions 5 07-25-2011 06:36 AM
Introducing our Ion Torrent! nickloman Ion Torrent 34 05-26-2011 06:56 PM
Ion Torrent in Cambridge! asr Ion Torrent 6 03-17-2011 12:32 PM

Reply
 
Thread Tools
Old 06-09-2011, 03:11 AM   #1
brianlamx
Member
 
Location: Cambridge, UK

Join Date: Jun 2009
Posts: 17
Default Ion Torrent, how much to run?

Does anyone have an idea on roughly how much an ion torrent instrument costs and how expensive it is to run?
brianlamx is offline   Reply With Quote
Old 06-09-2011, 09:08 AM   #2
genseq
Member
 
Location: Russia

Join Date: Dec 2007
Posts: 87
Default Costs

http://molbiol.ru/forums/index.php?a...post&id=112976
genseq is offline   Reply With Quote
Old 06-09-2011, 09:12 AM   #3
brianlamx
Member
 
Location: Cambridge, UK

Join Date: Jun 2009
Posts: 17
Default

good stuff! Thanks!
brianlamx is offline   Reply With Quote
Old 06-09-2011, 09:24 AM   #4
Hiro.Protagonist
Junior Member
 
Location: Cambridge, MA

Join Date: Jun 2011
Posts: 9
Default

The full cost depends on what support instrumentation you have available for the library prep and instrument prep.

From Life for the PGM and needed server plan for ~$80K

Ancillary equipment, like pH meter, water system, argon tanks, bioanalyzer, bioruptor, pippen prep, qubit, guava, thermal cycler, etc. that you may or may not need for library prep should fall into your budget and can cost more than the PGM.'

Running the instrument with the 314 chip costs ~>$1K per sample with their kits, chip, and consumables needed for the preps. Their stated run cost and run cost in that paper are for the superficial chip and reagents and not the inclusive cost you will pay.
Hiro.Protagonist is offline   Reply With Quote
Old 06-09-2011, 10:29 AM   #5
krobison
Senior Member
 
Location: Boston area

Join Date: Nov 2007
Posts: 747
Default

$49K for the box
$16K for the required Linux server
$5K for the upcoming emPCR device (not strictly required, but probably worth spending on)

List prices on reagents:

Cost for sample prep kits
RNA-Seq kit is $1.2K for 10 rxns (it's going to be a while before the instrument has the horsepower for most RNA-Seq projects, but perhaps microRNAs can work)

Fragment library kit is $500 to produce 10 libraries


Cost for template prep kit
$1250 for 10 samples

Sequencing kit
$600 for 8 reactions

Sequencing chip
314 $2000 for 8 chips
316 $4000 for 8 chips (supposedly not out 'til next month, but I can get a price on the website)

Cost for control library kit
$500 for 3 runs
(this is something it is probably wise to run periodically, but isn't strictly required)

So, per library for fragment libraries would be
$50 for fragment library
$125 for template prep
$75 for sequencing reagents
$250 for 314 chip

Or a total of $500 for the 314 chip and $750 for the 316 chip

(by which, with great effort, I get the same value as the review linked to above!)
krobison is offline   Reply With Quote
Old 06-09-2011, 10:48 AM   #6
Hiro.Protagonist
Junior Member
 
Location: Cambridge, MA

Join Date: Jun 2011
Posts: 9
Default

With you doing your work at an outside provider, did they charge $500 sample to do the sequencing? My only point would be to plan for more cost per sample than the actual kit components from Life, as they do not give you all you need to make the libraries. If you have to account for sample prep time for a staff member, that is a big portion of the cost involved with the PGM workflow.
Hiro.Protagonist is offline   Reply With Quote
Old 06-09-2011, 12:32 PM   #7
krobison
Senior Member
 
Location: Boston area

Join Date: Nov 2007
Posts: 747
Default

The costs I listed above would be if had an instrument on site, which is what the original question was about Yes, outside provider is $1250 (EdgeBio) for the 314 chip. And yes, the cost estimate did assume a certain level of basic laboratory setup (an argon tank is required to run the system). If someone was really starting a lab from scratch, then they would need to go through the protocols and tally up all that gear.
krobison is offline   Reply With Quote
Old 06-09-2011, 03:46 PM   #8
SeqAA
Guest
 

Posts: n/a
Default

Quote:
Originally Posted by Hiro.Protagonist View Post
The full cost depends on what support instrumentation you have available for the library prep and instrument prep.

From Life for the PGM and needed server plan for ~$80K

Ancillary equipment, like pH meter, water system, argon tanks, bioanalyzer, bioruptor, pippen prep, qubit, guava, thermal cycler, etc. that you may or may not need for library prep should fall into your budget and can cost more than the PGM.'

Running the instrument with the 314 chip costs ~>$1K per sample with their kits, chip, and consumables needed for the preps. Their stated run cost and run cost in that paper are for the superficial chip and reagents and not the inclusive cost you will pay.

You must work for illumina marketing.
  Reply With Quote
Old 06-09-2011, 03:59 PM   #9
ECO
--Site Admin--
 
Location: SF Bay Area, CA, USA

Join Date: Oct 2007
Posts: 1,352
Default

Quote:
Originally Posted by SeqAA View Post
You must work for illumina marketing.
Ad hominem. Don't do it, please.
ECO is offline   Reply With Quote
Old 06-17-2011, 06:48 PM   #10
Hiro.Protagonist
Junior Member
 
Location: Cambridge, MA

Join Date: Jun 2011
Posts: 9
Default

No, I think Illumina is equally as guilty at stating run costs but not the real cost. "Reagent cost" of a genome, for example.

It is fair that I exaggerated the cost, my only point is the cost to run isn't just the kits from any manufacturer, be it PGM or MiSeq. If you need to budget, adding a factor for all the other things you need to actually do a run is fair.

I would say it is not marketing, just reality. Add a similar factor for anything Illumina or others say about cost per run.

Also a run for any is not going to be 100% successful, in library prep, emPCR, cluster generation, sequencing, etc. Average out your success rate and the cost per sample will also be a bit higher.
Hiro.Protagonist is offline   Reply With Quote
Old 08-10-2011, 07:25 PM   #11
larissa
Member
 
Location: NC

Join Date: Mar 2010
Posts: 15
Default

Could someone please clarify what is the referred guava for the Ion? Because I am exactly going through protocols to tally up everything needed to set up the system and do not remember reading about it. Thanks in advance!
larissa is offline   Reply With Quote
Old 08-11-2011, 03:33 PM   #12
SeqAA
Guest
 

Posts: n/a
Default

There is no guava.. This has been replaced by an optional qubit assay.
  Reply With Quote
Old 08-14-2011, 07:21 PM   #13
lek2k
Member
 
Location: Australia

Join Date: Aug 2011
Posts: 32
Default

Two things to note since krobinson posted in June.

1. The Q3 price has changed, most notable the 314 chips are $100 dollars which means $800 for a pack of 8. The price for the 314 chip is exactly the same in Australia for once !!

2. Prices vary locally, thus converting it to British pounds may not be an indicator of price. krobinson I assume has quoted the US price. In Australia we pay 20% more than the US, despite the AUD being stronger for the whole year. Previously someone from the UK complained that they had to pay more than their US colleagues.
lek2k is offline   Reply With Quote
Old 08-14-2011, 07:31 PM   #14
lek2k
Member
 
Location: Australia

Join Date: Aug 2011
Posts: 32
Default

Comment regarding Ancillary equipment.
You do not need a pH meter, the PGM now does all the pH for you.

Bioruptor not required. The latest library prep kit uses enzymatic digestion (Ion Shear). This is a relief since the Bioruptor is quite expensive for a sonicator. In fact, all DNA sonicators are quite expensive e.g. in Australia a Covaris S2 costs more than the Ion Torrent itself!!

We are trialing the Invitrogen E-gel size select system as a cheaper alternative to pippen prep.
lek2k is offline   Reply With Quote
Old 08-24-2011, 11:48 PM   #15
halpink
Junior Member
 
Location: Singapore

Join Date: Aug 2011
Posts: 1
Default

How is the Invitrogen E-gel size select working out, lek2k?
halpink is offline   Reply With Quote
Old 08-25-2011, 12:30 AM   #16
lek2k
Member
 
Location: Australia

Join Date: Aug 2011
Posts: 32
Default

Had a go last week. Didn't go so good for the 2% size select gel
1. The gel had to be run much longer than in the instructions.
2. The water you put in the collection slots disappears quite fast. Don't puncture the gel and the bottom of the slot or there will be no collection in that slot.
3. After the selection the 260/280 ratio from the nanodrop was not so good.
4. Bioanalyzer showing size distribution result could be better.

Mind you this is the first time we have tried so we will persist cuz I like the idea and its cheap. Once we get better then we can whine about it. This was with NEB fragmentase, next try will be with the Ion Shear reagents.
lek2k is offline   Reply With Quote
Old 08-30-2011, 06:17 AM   #17
dongzw
Junior Member
 
Location: Shanghai

Join Date: Aug 2011
Posts: 8
Thumbs up

Quote:
Originally Posted by genseq View Post
Thank you very much, for the valuble paper
dongzw is offline   Reply With Quote
Old 08-30-2011, 12:21 PM   #18
DNADEB
Member
 
Location: PA

Join Date: Feb 2009
Posts: 29
Default

Our charge for one sample on a 316 chip is $1600. That includes library prep, size selection on a pippin (works great!), bioanalyzer chip to check libraries, consumables but no service contract yet. Shearing either by covaris or enzymatic process. We are counting on the OneTouch to lower labor costs. Right now, you have to buy an IKA for emulsifier step. Also, library prep uses more AMPure beads than other NextGen processes which was a surprise to us.
DNADEB is offline   Reply With Quote
Old 08-31-2011, 03:12 PM   #19
lek2k
Member
 
Location: Australia

Join Date: Aug 2011
Posts: 32
Default

People, we need to say what the local currency is. There is still a massive price difference after currency conversion.
lek2k is offline   Reply With Quote
Reply

Thread Tools

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off




All times are GMT -8. The time now is 01:58 AM.


Powered by vBulletin® Version 3.8.9
Copyright ©2000 - 2017, vBulletin Solutions, Inc.
Single Sign On provided by vBSSO