Seqanswers Leaderboard Ad

Collapse

Announcement

Collapse
No announcement yet.
X
 
  • Filter
  • Time
  • Show
Clear All
new posts

  • Segmentation fault (core dumped) at contig step during SOAP denovo assembly

    Hi all,
    I intend to assembly a 2.5G-sized genome with 2.4 G shut gun reads (100 bp) and 2.9 G mate-paried reads (75 bp). I run the SOAP denovo (v 1.05) step by step. The first step, pregraph, succeed in running. However, at the second step, the contig step, SOAP denovo reported the error message. The command line and error message are shown as below:

    SOAPdenovo-31mer pregraph -s shutgun_Q.gPET_Q.SOAP.kmer_21.config_1 -K 21 -a 650 -p 40 -R -o shutgun_Q.gPET_Q.K21

    SOAPdenovo-31mer contig -g shutgun_Q.gPET_Q.K21 -R
    Version 1.05: released on July 29th, 2010

    there're 415826527 kmers in vertex file
    there're 1447198408 edge in edge file
    done sort
    input 1447198408 edges
    1976892894 pre-arcs loaded
    66317675738 markers overall
    66317675738 markers loaded
    11919701 repeats solvable, 23896696 more edges
    47793392 dead arcs removed
    time spent on solving repeat: 53867s
    start to pinch bubbles, cutoff 0.100000, MAX NODE NUM 3, MAX DIFF 2
    Segmentation fault (core dumped)

    I am eagerly looking forward for your help.


    Yours,

    Zhonghui

  • #2
    Hi Zhonghui,

    Did you find a solution to this, as am having the same problems....

    Comment

    Latest Articles

    Collapse

    • seqadmin
      Recent Advances in Sequencing Analysis Tools
      by seqadmin


      The sequencing world is rapidly changing due to declining costs, enhanced accuracies, and the advent of newer, cutting-edge instruments. Equally important to these developments are improvements in sequencing analysis, a process that converts vast amounts of raw data into a comprehensible and meaningful form. This complex task requires expertise and the right analysis tools. In this article, we highlight the progress and innovation in sequencing analysis by reviewing several of the...
      05-06-2024, 07:48 AM
    • seqadmin
      Essential Discoveries and Tools in Epitranscriptomics
      by seqadmin




      The field of epigenetics has traditionally concentrated more on DNA and how changes like methylation and phosphorylation of histones impact gene expression and regulation. However, our increased understanding of RNA modifications and their importance in cellular processes has led to a rise in epitranscriptomics research. “Epitranscriptomics brings together the concepts of epigenetics and gene expression,” explained Adrien Leger, PhD, Principal Research Scientist...
      04-22-2024, 07:01 AM

    ad_right_rmr

    Collapse

    News

    Collapse

    Topics Statistics Last Post
    Started by seqadmin, 05-10-2024, 06:35 AM
    0 responses
    18 views
    0 likes
    Last Post seqadmin  
    Started by seqadmin, 05-09-2024, 02:46 PM
    0 responses
    21 views
    0 likes
    Last Post seqadmin  
    Started by seqadmin, 05-07-2024, 06:57 AM
    0 responses
    19 views
    0 likes
    Last Post seqadmin  
    Started by seqadmin, 05-06-2024, 07:17 AM
    0 responses
    21 views
    0 likes
    Last Post seqadmin  
    Working...
    X