Dear all,
I would like to know if it is possible to do a differential expression analysis on samples that have different replicate numbers?
I recently received my data using illumina hi-seq for bacteria samples. I had 3 replicates per sample but when I did the alignment on bowtie2 1 sample had a replicate with only 49% alignment rate.
Does anyone have suggestion on how to proceed? I am thinking of just doing a 3 replicate sample A vs 2 replicate sample B. Is this acceptable or should I adjust it to 2 replicates per sample?
Thank you for your answer.
Jason
I would like to know if it is possible to do a differential expression analysis on samples that have different replicate numbers?
I recently received my data using illumina hi-seq for bacteria samples. I had 3 replicates per sample but when I did the alignment on bowtie2 1 sample had a replicate with only 49% alignment rate.
Does anyone have suggestion on how to proceed? I am thinking of just doing a 3 replicate sample A vs 2 replicate sample B. Is this acceptable or should I adjust it to 2 replicates per sample?
Thank you for your answer.
Jason
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