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Old 06-17-2020, 07:54 AM   #1
Location: Hyderabad, India

Join Date: Apr 2010
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Default Minimum transcriptome requirement for genome annotation


I would like to know if there is any information available on the minimum number of transcriptomes required to get a genome annotation that predicts 90% of the genes. For example, If you are building a genome annotation (specifically for gene) for a new species, and would like to generate a reasonably comprehensive annotation in that species (90% of the genes and gene models captured), then how many transcriptomes would help you?

I am thinking 3 tissues x 3 developmental stages, so 9 transcriptomes. Does anyone have a different suggestion on how to approach this? or a publication you can point me to?

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Old 06-18-2020, 08:18 PM   #2
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Location: US

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For a plant or an animal?
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annotation, rna-sequencing, transcriptome

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