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Old 10-22-2015, 03:33 AM   #1
soleulloa
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Location: Chile

Join Date: Aug 2011
Posts: 33
Default Tween 20% affects Flow cell ??

Hi,
I have some problems in pre-check of a run and MiSeq can't initiate correctly by a flow rate error.
I put reagents at 4C and I did a wash using H2O+Tween 20% for clearing lines, but I forgot to change the new flow cell by another.

So, my flow cell was washed using Tween 20% before the run... it affects flow cell? Could tween 20 remove the oligos used for bridge PCR and clustering?
Maybe I need to change the flow cell?

Help Me!

Soledad
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