Seqanswers Leaderboard Ad

Collapse

Announcement

Collapse
No announcement yet.
X
 
  • Filter
  • Time
  • Show
Clear All
new posts

  • bismark - fasta alignment result?

    Hi.
    I used 'bismark' to align bisulfite treated DNA sequences in the fasta format.
    Bismark produced a .sam and a report file along with a overview pic.
    When I opened the .sam file, it didn't contain any information about aligned sequence. So, I couldn't figure out which sequences were aligned. Please, somebody tell me how to get them.

  • #2
    1. How did you open the SAM file.
    2. What did the SAM file contain?

    It's likely that you were just looking at the header and needed to scroll down.

    Comment


    • #3
      I opened the SAM files using 'less' command and tried to scrolled down to the end of the file. But the sam file contained only the header and nothing else.

      Thanks, dpryan!

      Comment


      • #4
        Presumably you got an error at some point then. You'll have to find it and figure out why that happened.

        Comment


        • #5
          If you could send the the onscreen text you see when running your FastA file via email I could try to help.

          Comment

          Latest Articles

          Collapse

          • seqadmin
            Essential Discoveries and Tools in Epitranscriptomics
            by seqadmin


            The field of epigenetics has traditionally concentrated more on DNA and how changes like methylation and phosphorylation of histones impact gene expression and regulation. However, our increased understanding of RNA modifications and their importance in cellular processes has led to a rise in epitranscriptomics research. “Epitranscriptomics brings together the concepts of epigenetics and gene expression,” explained Adrien Leger, PhD, Principal Research Scientist on Modified Bases...
            Yesterday, 07:01 AM
          • seqadmin
            Current Approaches to Protein Sequencing
            by seqadmin


            Proteins are often described as the workhorses of the cell, and identifying their sequences is key to understanding their role in biological processes and disease. Currently, the most common technique used to determine protein sequences is mass spectrometry. While still a valuable tool, mass spectrometry faces several limitations and requires a highly experienced scientist familiar with the equipment to operate it. Additionally, other proteomic methods, like affinity assays, are constrained...
            04-04-2024, 04:25 PM

          ad_right_rmr

          Collapse

          News

          Collapse

          Topics Statistics Last Post
          Started by seqadmin, 04-11-2024, 12:08 PM
          0 responses
          39 views
          0 likes
          Last Post seqadmin  
          Started by seqadmin, 04-10-2024, 10:19 PM
          0 responses
          41 views
          0 likes
          Last Post seqadmin  
          Started by seqadmin, 04-10-2024, 09:21 AM
          0 responses
          35 views
          0 likes
          Last Post seqadmin  
          Started by seqadmin, 04-04-2024, 09:00 AM
          0 responses
          55 views
          0 likes
          Last Post seqadmin  
          Working...
          X