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  • Library prep for 50ng input with TruSeq DNA- or RNAseq adapters?

    Dear all,

    I am going to process library preparation with 50 ng DNA. I am just concerning ratio of adaptersNA input. Thus, I am considering to use adapters from TruSeq RNAseq kit, which has lower molarity adapters. Has anyone done library prep with lower DNA input?

  • #2
    Yes or you could just dilute your DNA TruSeq adapters 20x.

    We have gone below 1 ug. Possibly to 100 ng, with no modifications to the protocol. But we generally size select prior to enrichment PCR. So adapter dimers are not that much of an issue.

    --
    Phillip

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