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I'd like to know what types of ranges people are getting for the total quantity of their final chip-seq and/or msp1-digested rrbs libraries. For chip-seq, let us assume the starting amount of DNA is 1-10ng and 15-17 cycles of post-ligation PCR with phusion taq. For rrbs let us assume the starting amount of genomic DNA is 500ng also with the same range of PCR (using Pfu Turbo Cx taq from Agilent) post-ligation and bisulfite conversion.
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The sequencing world is rapidly changing due to declining costs, enhanced accuracies, and the advent of newer, cutting-edge instruments. Equally important to these developments are improvements in sequencing analysis, a process that converts vast amounts of raw data into a comprehensible and meaningful form. This complex task requires expertise and the right analysis tools. In this article, we highlight the progress and innovation in sequencing analysis by reviewing several of the...05-06-2024, 07:48 AM -
The field of epigenetics has traditionally concentrated more on DNA and how changes like methylation and phosphorylation of histones impact gene expression and regulation. However, our increased understanding of RNA modifications and their importance in cellular processes has led to a rise in epitranscriptomics research. “Epitranscriptomics brings together the concepts of epigenetics and gene expression,” explained Adrien Leger, PhD, Principal Research Scientist...04-22-2024, 07:01 AM
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