Go Back   SEQanswers > Applications Forums > Sample Prep / Library Generation

Similar Threads
Thread Thread Starter Forum Replies Last Post
QIAseq targeted DNA Panel Question HelenaSC Sample Prep / Library Generation 12 10-08-2018 05:11 AM
QIAseq 1-Step Amplicon Library kit-reviews? Buckethead84 Sample Prep / Library Generation 1 09-09-2017 02:28 AM
how to compute all restriction enzyme sites in the human genome? lunacab General 10 07-09-2017 03:36 PM
Optimizing magnetic bead mix for gDNA extraction anjama Sample Prep / Library Generation 3 07-04-2017 02:51 PM
Control libraries from Human gDNA andymay Illumina/Solexa 0 07-22-2013 01:28 PM

Thread Tools
Old 10-11-2017, 01:35 PM   #1
Junior Member
Location: SLC

Join Date: Jan 2012
Posts: 1
Default QIAseq FX Enzyme Mix (for human gDNA)

Exploring the possibility of using this kit for our hyb-capture library prep front-end process. Does anyone know the specific enzymes that make up the cocktails? As well, is the enzymatic fragmentation random or does it target specific sequences? Is it known what the fragmentation enzymes are? Thanks
JWL is offline   Reply With Quote
Old 10-12-2017, 01:02 PM   #2
Senior Member
Location: CT

Join Date: Apr 2015
Posts: 233

My Qiagen rep was just here talking about this. He claimed that it isn't sequence specific cleaving at all but beyond that I couldn't get much info out of him. Showed some data comparing sequencing sites to covaris sheared DNA. They didn't have any company data on what I'd call high gc genomes so he's going send me a kit to try on some 75% GC streptococcus
Microbial ecologist, running a sequencing core. I have lots of strong opinions on how to survey communities, pretty sure some are even correct.
thermophile is offline   Reply With Quote

Thread Tools

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off

All times are GMT -8. The time now is 03:20 PM.

Powered by vBulletin® Version 3.8.9
Copyright ©2000 - 2018, vBulletin Solutions, Inc.
Single Sign On provided by vBSSO