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Old 10-02-2017, 03:39 PM   #1
hewenjuan
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Default Oligo dT VN

Oligo dT VN is used for anchored cDNA synthesis. But what does "VN" in the end do? Please help. Thanks.
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Old 10-03-2017, 03:59 AM   #2
nucacidhunter
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Oligo dT length is normally 30nt but transcript poly A tail could be longer. Including VN at 3 end of RT primer enriches for non-poly A region of transcripts and without it RT can be primed in poly A region so cDNA would have A/T homopolymer which in most sequencing applications is undesirable. A side effect of VN inclusion is possible bias in some transcripts conversion to cDNA.
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Old 10-17-2017, 06:34 AM   #3
Jimbu
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'N' is any base, 'V' is either A,C or G. This allows for anchoring at the beginning of the Poly-A tail.
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