We bought this kit for DNAseq from Agilent (Haloplex Target Enrichment System) to use in Illumina Miseq. Right now, we have DNA Ligase (that came with the kit), for 5 reaction (samples preparation). If we had another tube of DNA Ligase, we would be able to raise up to 36 reactions, since we still have the other reagents sufficient for 36 reactions...
I saw on Agilent's website that they sell two different types of DNA Ligase: The PFU DNA Ligase and the T4 DNA Ligase.
I would like to know if somebody already tried to use one of these DNA Ligase in the kit to verify if works?
Thanks in advance...
I saw on Agilent's website that they sell two different types of DNA Ligase: The PFU DNA Ligase and the T4 DNA Ligase.
I would like to know if somebody already tried to use one of these DNA Ligase in the kit to verify if works?
Thanks in advance...
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