Seqanswers Leaderboard Ad

Collapse

Announcement

Collapse
No announcement yet.
X
 
  • Filter
  • Time
  • Show
Clear All
new posts

  • nrdb program

    Hi there,
    Does anyone know a link to download the nrdb program that removes redundancy from a file of sequences? I looked around the NCBI but did not actually find it.

    If anyone has a copy or knows a link to one, it will be most appreciated.

    Thank you

  • #2
    Originally posted by poisson200 View Post
    Does anyone know a link to download the nrdb program that removes redundancy from a file of sequences? I looked around the NCBI but did not actually find it.
    It comes with the WU-BLAST distribution: http://blast.wustl.edu/

    The EBI Exonerate software comes with "fastanrdb" equivalent: http://www.ebi.ac.uk/~guy/exonerate/

    BioPerl comes with a "bp_nrdb.pl" equivalent: http://bioperl.org/

    Comment


    • #3
      Great, thank you

      Dear Torst.
      That is great. I knew about Wublast but if you check their website, it has been handed over to a company that now charge all to use it.

      But fastanrdb sounds great.

      Thank you.

      John.

      Comment


      • #4
        Originally posted by poisson200 View Post
        That is great. I knew about Wublast but if you check their website, it has been handed over to a company that now charge all to use it.
        Yes I know, but you can get a personal licence for free: http://blast.advbiocomp.com/licensing/

        As you only want "nrdb" (assuming it is still part of the package) you won't be violating any "publish results of" clauses?

        Comment


        • #5
          nrdb freely available from AB-BLAST web site

          While nrdb 3.0 was bundled with the last version of WU-BLAST (and presumably is with AB-BLAST). nrdb 2 was also freely available (i.e. not requiring a WU-BLAST license) as a separate download from the web site. Checking on the AB-BLAST web site this is still the case, so you can download pre-built executables or the source for nrdb from:

          Comment

          Latest Articles

          Collapse

          • seqadmin
            Current Approaches to Protein Sequencing
            by seqadmin


            Proteins are often described as the workhorses of the cell, and identifying their sequences is key to understanding their role in biological processes and disease. Currently, the most common technique used to determine protein sequences is mass spectrometry. While still a valuable tool, mass spectrometry faces several limitations and requires a highly experienced scientist familiar with the equipment to operate it. Additionally, other proteomic methods, like affinity assays, are constrained...
            04-04-2024, 04:25 PM
          • seqadmin
            Strategies for Sequencing Challenging Samples
            by seqadmin


            Despite advancements in sequencing platforms and related sample preparation technologies, certain sample types continue to present significant challenges that can compromise sequencing results. Pedro Echave, Senior Manager of the Global Business Segment at Revvity, explained that the success of a sequencing experiment ultimately depends on the amount and integrity of the nucleic acid template (RNA or DNA) obtained from a sample. “The better the quality of the nucleic acid isolated...
            03-22-2024, 06:39 AM

          ad_right_rmr

          Collapse

          News

          Collapse

          Topics Statistics Last Post
          Started by seqadmin, 04-11-2024, 12:08 PM
          0 responses
          30 views
          0 likes
          Last Post seqadmin  
          Started by seqadmin, 04-10-2024, 10:19 PM
          0 responses
          32 views
          0 likes
          Last Post seqadmin  
          Started by seqadmin, 04-10-2024, 09:21 AM
          0 responses
          28 views
          0 likes
          Last Post seqadmin  
          Started by seqadmin, 04-04-2024, 09:00 AM
          0 responses
          53 views
          0 likes
          Last Post seqadmin  
          Working...
          X