Hi, all,
With the previous version of CASAVA, one first had to do conversion of bcl to qseq files and then convert qseq to fastq getting separate fastq files: one with reads (two for paired end runs) and another one for barcodes. Next step was demultiplex your data using all these fastq-files.
New version of CASAVA directly converts bcl files into fastq and perform demultiplexing in the same time. Is it possible to extract barcode fastq files? I have not found how to do it in the manual provided for bcltofactqconversion script.
The thing is that, results of my last sequencing run looks quite weird and most reads can not be assign to a specific library, so I would like to have a look on the barcodes separately and check the sequencing quality.
Will be very grateful for any suggestions.
With the previous version of CASAVA, one first had to do conversion of bcl to qseq files and then convert qseq to fastq getting separate fastq files: one with reads (two for paired end runs) and another one for barcodes. Next step was demultiplex your data using all these fastq-files.
New version of CASAVA directly converts bcl files into fastq and perform demultiplexing in the same time. Is it possible to extract barcode fastq files? I have not found how to do it in the manual provided for bcltofactqconversion script.
The thing is that, results of my last sequencing run looks quite weird and most reads can not be assign to a specific library, so I would like to have a look on the barcodes separately and check the sequencing quality.
Will be very grateful for any suggestions.
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