Hey Everyone,
I'm trying to make libraries from S2 cells and have a question regarding what the bioanalyzer electropherogram should look like. I know the RIN numbers for insect RNA are usually pretty low due to the dissociation of the 28S rRNA, but I can't seem to find any examples of "good" insect RNA for a visual comparison. I'm using the pico chip, RIN numbers are between 6-7.5, samples are not heat denatured prior to loading. Thanks!
I'm trying to make libraries from S2 cells and have a question regarding what the bioanalyzer electropherogram should look like. I know the RIN numbers for insect RNA are usually pretty low due to the dissociation of the 28S rRNA, but I can't seem to find any examples of "good" insect RNA for a visual comparison. I'm using the pico chip, RIN numbers are between 6-7.5, samples are not heat denatured prior to loading. Thanks!
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