Hello everyone,
I mapped my Solid data with Bioscope. In the csfasta files are around 142 million reads. This value is shown in the pairing statistics file as well. But in the bam file with the mapped and unmapped reads are together just 119 million reads. I checked that with the help of samtools. What happened to the other reads? Does Bioscope filter any reads? I couldn't find anything about that.
Furthermore the pairing statistics show more mapped pairs than I could find in the bam file. Could anybody explain that, please?
I would be very happy about some help or comments. Thanks
I mapped my Solid data with Bioscope. In the csfasta files are around 142 million reads. This value is shown in the pairing statistics file as well. But in the bam file with the mapped and unmapped reads are together just 119 million reads. I checked that with the help of samtools. What happened to the other reads? Does Bioscope filter any reads? I couldn't find anything about that.
Furthermore the pairing statistics show more mapped pairs than I could find in the bam file. Could anybody explain that, please?
I would be very happy about some help or comments. Thanks
Comment