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  • Segmentation fault (core dumped) at contig step during SOAP denovo assembly

    Hi all,
    I intend to assembly a 2.5G-sized genome with 2.4 G shut gun reads (100 bp) and 2.9 G mate-paried reads (75 bp). I run the SOAP denovo (v 1.05) step by step. The first step, pregraph, succeed in running. However, at the second step, the contig step, SOAP denovo reported the error message. The command line and error message are shown as below:

    SOAPdenovo-31mer pregraph -s shutgun_Q.gPET_Q.SOAP.kmer_21.config_1 -K 21 -a 650 -p 40 -R -o shutgun_Q.gPET_Q.K21

    SOAPdenovo-31mer contig -g shutgun_Q.gPET_Q.K21 -R
    Version 1.05: released on July 29th, 2010

    there're 415826527 kmers in vertex file
    there're 1447198408 edge in edge file
    done sort
    input 1447198408 edges
    1976892894 pre-arcs loaded
    66317675738 markers overall
    66317675738 markers loaded
    11919701 repeats solvable, 23896696 more edges
    47793392 dead arcs removed
    time spent on solving repeat: 53867s
    start to pinch bubbles, cutoff 0.100000, MAX NODE NUM 3, MAX DIFF 2
    Segmentation fault (core dumped)

    I am eagerly looking forward for your help.


    Yours,

    Zhonghui

  • #2
    Hi Zhonghui,

    Did you find a solution to this, as am having the same problems....

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