hi!
sorry in advance for this stupid question, but i couldn´t figure it out anywhere:
what is 1/2 (or 1/8) run? does this only mean that half of the standard-time is used for sequencing, so providing just half of the data? and how is this related to the coverage? is coverage only a function of genome-size and sequenced megabases? so can i calculate the estimated-coverage out of the genome-size and the estimated totally-sequenced-megabases (depending on the number of runs and the 454-system used)?
i hope someone can help me, because this is confunsing me a bit!
many thanks. Triticum.
sorry in advance for this stupid question, but i couldn´t figure it out anywhere:
what is 1/2 (or 1/8) run? does this only mean that half of the standard-time is used for sequencing, so providing just half of the data? and how is this related to the coverage? is coverage only a function of genome-size and sequenced megabases? so can i calculate the estimated-coverage out of the genome-size and the estimated totally-sequenced-megabases (depending on the number of runs and the 454-system used)?
i hope someone can help me, because this is confunsing me a bit!
many thanks. Triticum.
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