Hello to all,
I get some errors with the bclToFastq tool, of type:
But when I do, for example:
The file exists.
Someone has already encountered this problem? My bcl file has not been modified, it comes directly from Illumina sequencer. On the sequencer, the data are of good quality and have been correctly demultiplexed. As I understand it, the getCluster function is empty, yet my file is not empty ...
I have never encountered this problem. My samples are multiplexed on multiple lanes, so the bcltofastq tool must pool them. Yet I have done this several times, no problem. To make the samples pool, the bcltofastq tool is based on sample names in the samplesheet?
Thanks in advance.
I get some errors with the bclToFastq tool, of type:
[2014-12-17 14:41:54] [lame26] [Temp/L006_R1_demux_summary.xml] Error: 2014-Dec-17 14:41:54: Success: CASAVA_v1.8.2/src/c++/lib/alignment/BclReader.cpp(148): Throw in function void casava::alignment::BclReader::getCluster(std::string&, std::string&)
[2014-12-17 14:41:54] [lame26] [Temp/L006_R1_demux_summary.xml] Dynamic exception type: boost::exception_detail::clone_impl<casava::common::IoException>
[2014-12-17 14:41:54] [lame26] [Temp/L006_R1_demux_summary.xml] std::exception::what: Failed to read BCL file Data/Intensities/BaseCalls/L006/C53.1/s_6_1107.bcl.
[2014-12-17 14:41:54] [lame26] [Temp/L006_R1_demux_summary.xml] : Failed to read BCL file Data/Intensities/BaseCalls/L006/C53.1/s_6_1107.bcl.
make: *** [Temp/L006_R1_demux_summary.xml] Error 1
[2014-12-17 14:41:54] [lame26] [Temp/L006_R1_demux_summary.xml] Dynamic exception type: boost::exception_detail::clone_impl<casava::common::IoException>
[2014-12-17 14:41:54] [lame26] [Temp/L006_R1_demux_summary.xml] std::exception::what: Failed to read BCL file Data/Intensities/BaseCalls/L006/C53.1/s_6_1107.bcl.
[2014-12-17 14:41:54] [lame26] [Temp/L006_R1_demux_summary.xml] : Failed to read BCL file Data/Intensities/BaseCalls/L006/C53.1/s_6_1107.bcl.
make: *** [Temp/L006_R1_demux_summary.xml] Error 1
But when I do, for example:
ls Data/Intensities/BaseCalls/L006/C53.1/s_6_1107.bcl
Someone has already encountered this problem? My bcl file has not been modified, it comes directly from Illumina sequencer. On the sequencer, the data are of good quality and have been correctly demultiplexed. As I understand it, the getCluster function is empty, yet my file is not empty ...
I have never encountered this problem. My samples are multiplexed on multiple lanes, so the bcltofastq tool must pool them. Yet I have done this several times, no problem. To make the samples pool, the bcltofastq tool is based on sample names in the samplesheet?
Thanks in advance.
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