T over-representation in NextSeq500 run

Asashoryu · 01-11-2018, 04:18 AM

Hello, we got strange results from some of the recent RNA-seq runs on NextSeq500; example: image001.png

Funny is that we have observed it in some runs while other runs from the same experiment (all processed with NextFlex kit and after polyA selection) but sequenced later worked fine. (RIN was ok, no weird yield after library preparation)

Illumina blaims the sample... What makes sense as the re-run with flowcell from different batch showed same result. But I do not understand what would make such a strange result.

Any thoughts?

Similar Threads
Thread	Thread Starter	Forum	Replies	Last Post
NextSeq500 early results	HMorrison	Illumina/Solexa	6	07-22-2015 02:29 PM
nextSeq500 heat output	RemitoAmigo	Illumina/Solexa	1	06-04-2015 12:03 PM
Nextseq500 Super Wash	cnicolet	Illumina/Solexa	2	02-27-2015 10:13 AM
Nextseq500 N's from position 25	Asaf	Illumina/Solexa	2	02-23-2015 02:52 AM
Exome Sequencing on NextSeq500	sfranzenburg	Illumina/Solexa	2	11-17-2014 06:12 AM

01-11-2018, 04:18 AM	#1
Asashoryu Junior Member Location: NL Join Date: May 2009 Posts: 7	T over-representation in NextSeq500 run Hello, we got strange results from some of the recent RNA-seq runs on NextSeq500; example: image001.png Funny is that we have observed it in some runs while other runs from the same experiment (all processed with NextFlex kit and after polyA selection) but sequenced later worked fine. (RIN was ok, no weird yield after library preparation) Illumina blaims the sample... What makes sense as the re-run with flowcell from different batch showed same result. But I do not understand what would make such a strange result. Any thoughts?