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Wiki News! dan Wiki Discussion 0 03-13-2010 08:59 AM

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Old 09-24-2018, 07:07 AM   #1
PerkinElmer AG
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Location: Austin, TX

Join Date: Jul 2018
Posts: 3
Default News of CRISPR's demise have been greatly exaggerated

Two recent articles suggest that CRISPR/Cas9 can cause mutations in the p53 pathway, irrespective of the target sequence of the guide RNA. The data are extremely interesting since they raise the issue of unpredictable off-target effects that are not directly introduced by the CRISPR/Cas9 complex but rather selected during the genome manipulation process. A thorough analysis of the genomic integrity of cells genetically manipulated with CRISPR is, therefore, the key to successful use of the technology, as described in https://perkinelmer-appliedgenomics.com/research-review-crispr-cas9/ .
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