Seqanswers Leaderboard Ad

Collapse

Announcement

Collapse
No announcement yet.
X
 
  • Filter
  • Time
  • Show
Clear All
new posts

  • Targeted RNASeq procotols for viral sequencing

    Hello,

    I am starting a project on which I would like to detect viral RNA (herpes) in human tissue. Essentially I would extract the RNA, perform targeted amplification and library prep for multiple viruses and then sequence. I think I have an idea of the bioinformatic system to use.
    However, I am not really sure where to start on the library prep issue (i.e. which library prep system to use). I've looked into ScripSeq which sounded promising but seems very expensive.

    Is there a "custom" protocol/template out there than I can use to accomplish these goals. My main concerns are (if i would to start with known herpes primers, how would I successfully make a library that contain adapters and index for multiplex?)

    Thank you and excuse my naive questions. I am not new to using NGS for already commercially prepared custom gene panels (i.e. Qiagen targeted RNASeq panels) but I am new in developing custom assays so your help would be greatly appreciated.

  • #2
    I found out what I needed if anyone is interested. It is called (16S Metagenomic sequencing library preparation). It is made for 16S rRNA targeted amplification, however the protocol can easily be adjusted for any targeted region on any genome. all you need is to attach illumina adapter overhang sequences to the targeting Forward and Reverse primers, amplify, clean up, amplify again with indexes, clean up and sequence.

    Comment

    Latest Articles

    Collapse

    • seqadmin
      Techniques and Challenges in Conservation Genomics
      by seqadmin



      The field of conservation genomics centers on applying genomics technologies in support of conservation efforts and the preservation of biodiversity. This article features interviews with two researchers who showcase their innovative work and highlight the current state and future of conservation genomics.

      Avian Conservation
      Matthew DeSaix, a recent doctoral graduate from Kristen Ruegg’s lab at The University of Colorado, shared that most of his research...
      03-08-2024, 10:41 AM
    • seqadmin
      The Impact of AI in Genomic Medicine
      by seqadmin



      Artificial intelligence (AI) has evolved from a futuristic vision to a mainstream technology, highlighted by the introduction of tools like OpenAI's ChatGPT and Google's Gemini. In recent years, AI has become increasingly integrated into the field of genomics. This integration has enabled new scientific discoveries while simultaneously raising important ethical questions1. Interviews with two researchers at the center of this intersection provide insightful perspectives into...
      02-26-2024, 02:07 PM

    ad_right_rmr

    Collapse

    News

    Collapse

    Topics Statistics Last Post
    Started by seqadmin, 03-14-2024, 06:13 AM
    0 responses
    33 views
    0 likes
    Last Post seqadmin  
    Started by seqadmin, 03-08-2024, 08:03 AM
    0 responses
    72 views
    0 likes
    Last Post seqadmin  
    Started by seqadmin, 03-07-2024, 08:13 AM
    0 responses
    81 views
    0 likes
    Last Post seqadmin  
    Started by seqadmin, 03-06-2024, 09:51 AM
    0 responses
    68 views
    0 likes
    Last Post seqadmin  
    Working...
    X