So Qiagen has this RNAprotect Cell Reagent (https://www.qiagen.com/us/shop/lab-b...inginformation) which pretty much sounds like the holy grail of RNA preservation within cells.
Does anybody understand (generally) how this reagent works? Does anybody know how to pellet cells suspended in it? Particularly how to pellet cells in reagent without lysing them? If I wash out the reagent will their transcripts remain preserved? Will my expression profiles really be representative of the cell in culture? Their protocol for using it only mentions processing bulk RNA extractions via their proprietary products (which makes no sense to me because why bother to preserve transcripts within the cells if you're going to run bulk extraction anyways?)
I'm asking because I need to optimize for single cell RNA seq (Drop Seq in particular) and I've already seen some basic anecdotal tests showing it's a miracle worker for preserving transcripts in trickier cell types.
I'm also curious if anyone has any experience using this prior to FACS or with dissociated tissue suspension preparations.
Does anybody understand (generally) how this reagent works? Does anybody know how to pellet cells suspended in it? Particularly how to pellet cells in reagent without lysing them? If I wash out the reagent will their transcripts remain preserved? Will my expression profiles really be representative of the cell in culture? Their protocol for using it only mentions processing bulk RNA extractions via their proprietary products (which makes no sense to me because why bother to preserve transcripts within the cells if you're going to run bulk extraction anyways?)
I'm asking because I need to optimize for single cell RNA seq (Drop Seq in particular) and I've already seen some basic anecdotal tests showing it's a miracle worker for preserving transcripts in trickier cell types.
I'm also curious if anyone has any experience using this prior to FACS or with dissociated tissue suspension preparations.
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