Hello all,
Have just run a 16S amplicon library on 454 Titanium. The enrichment, granted, was high (above 20%) but from 2E+6 raw wells only 36 sample key pass wells were identified with 0 control passes! The library worked perfectly before. My first assumption (as we are very sure we did nothing stupid) is reagents or kit - but how likely is this? Has anyone experienced something similar before or recently?
Many thanks
B
Have just run a 16S amplicon library on 454 Titanium. The enrichment, granted, was high (above 20%) but from 2E+6 raw wells only 36 sample key pass wells were identified with 0 control passes! The library worked perfectly before. My first assumption (as we are very sure we did nothing stupid) is reagents or kit - but how likely is this? Has anyone experienced something similar before or recently?
Many thanks
B
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