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  • Mapping transcriptome to genome

    I'm wondering about the best way to determine the proportion of different transcriptome assemblies (assembled in trinity) which can be mapped to a reference genome assembly - basically for an indication of completeness.

    I'm thinking that nucmer in the MUMer package might be a good place to start, with the transcripts as query and the genome as the reference sequence, but does anyone have a more cunning suggestion than that?

    Thanks in advance!

  • #2
    the Sim4db http://www.ncbi.nlm.nih.gov/pubmed/21551146 and Exonerate https://www.ebi.ac.uk/~guy/exonerate/beginner.html work good
    Last edited by GenoMax; 07-08-2015, 07:41 AM. Reason: fixed URL's

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