thanks in advance for answering
Seqanswers Leaderboard Ad
Collapse
Announcement
Collapse
No announcement yet.
X
-
When we started sequencing on the HiSeq2000 (september 2010), we had to perform 36nt run to be able to compare Hiseq2000 experiments and GAIIx experiments (read number is an issue but it's another subject).
It worked well and we didn't face any problem (in my memories) with the 2*72nt paired end run we performed with the remaining reagents.
-
Merci beaucoup!
I was recommended not to devide the 200 cycle into so many parts.
Originally posted by huguesparri View PostWhen we started sequencing on the HiSeq2000 (september 2010), we had to perform 36nt run to be able to compare Hiseq2000 experiments and GAIIx experiments (read number is an issue but it's another subject).
It worked well and we didn't face any problem (in my memories) with the 2*72nt paired end run we performed with the remaining reagents.
Comment
Latest Articles
Collapse
-
by seqadmin
Proteins are often described as the workhorses of the cell, and identifying their sequences is key to understanding their role in biological processes and disease. Currently, the most common technique used to determine protein sequences is mass spectrometry. While still a valuable tool, mass spectrometry faces several limitations and requires a highly experienced scientist familiar with the equipment to operate it. Additionally, other proteomic methods, like affinity assays, are constrained...-
Channel: Articles
04-04-2024, 04:25 PM -
-
by seqadmin
Despite advancements in sequencing platforms and related sample preparation technologies, certain sample types continue to present significant challenges that can compromise sequencing results. Pedro Echave, Senior Manager of the Global Business Segment at Revvity, explained that the success of a sequencing experiment ultimately depends on the amount and integrity of the nucleic acid template (RNA or DNA) obtained from a sample. “The better the quality of the nucleic acid isolated...-
Channel: Articles
03-22-2024, 06:39 AM -
ad_right_rmr
Collapse
News
Collapse
Topics | Statistics | Last Post | ||
---|---|---|---|---|
Started by seqadmin, 04-11-2024, 12:08 PM
|
0 responses
23 views
0 likes
|
Last Post
by seqadmin
04-11-2024, 12:08 PM
|
||
Started by seqadmin, 04-10-2024, 10:19 PM
|
0 responses
24 views
0 likes
|
Last Post
by seqadmin
04-10-2024, 10:19 PM
|
||
Started by seqadmin, 04-10-2024, 09:21 AM
|
0 responses
21 views
0 likes
|
Last Post
by seqadmin
04-10-2024, 09:21 AM
|
||
Started by seqadmin, 04-04-2024, 09:00 AM
|
0 responses
52 views
0 likes
|
Last Post
by seqadmin
04-04-2024, 09:00 AM
|
Comment