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Old 08-06-2009, 11:20 AM   #1
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Location: Kansas City

Join Date: Aug 2009
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Default A clarification for Illumina/Solexa Genome Analyzer Primer/Adapter Sequences

In the sticky thread "Illumina/Solexa Genome Analyzer Primer/Adapter Sequences", the 3rd reply of horigen tried to make people understand the sequence of adapter better, however, it misleaded people quite much. Actually, I spent almost 6 month to figure out it was totally wrong and find out the truth.
The Oligos provided by Illumina are just the right sequences, you need not order any more complemental oligo as horigen indicated.
The following figure is a schematic I made for preparing single-end sequence sample.
Wish not so many people spent the same time as me on the same mistake.
I also suggest delete the 3rd reply to prevent further misleading.
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File Type: jpg single-end.jpg (91.6 KB, 410 views)
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Old 08-06-2009, 06:57 PM   #2
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Hey kaichen,

What was the exact problem you encountered or what is wrong with horigen's post(s)? If there is indeed a problem it would be helpful to post within that sticky thread to make sure the problem is noted and corrected.

Your diagram seems to be missing 5' phosphates on the doesn't really make a difference as written but I figured you'd want to change it.
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