Dear all,
We are just about to use Truseq V3 SBS kit. We still not sure how much we should load. According to the protocol, it should be 20 pM for 825k/mm2, unlike V2 kit which recommends 8pM for 400 k/mm2. Has anyone titrated the cluster density?
We are just about to use Truseq V3 SBS kit. We still not sure how much we should load. According to the protocol, it should be 20 pM for 825k/mm2, unlike V2 kit which recommends 8pM for 400 k/mm2. Has anyone titrated the cluster density?