I am trying to supply TopHat with a GFF3 file of gene annotation, the pipeline will report the relative expression of each in the sample.
But I don't know why the <GFF3_file>.expr file is empty and the gff_juncs.log file reports the error message as the following
===========================================
Error: bad transcript annotation:
chr1 hg19_refFlat mRNA 24292939 24306821 . - . ID=FUSIP1;PARENT=FUSIP1;
Offending exons overlapped:
===========================================
Does anyone know the problem or meet the problem before?
Thanks a lot!
But I don't know why the <GFF3_file>.expr file is empty and the gff_juncs.log file reports the error message as the following
===========================================
Error: bad transcript annotation:
chr1 hg19_refFlat mRNA 24292939 24306821 . - . ID=FUSIP1;PARENT=FUSIP1;
Offending exons overlapped:
===========================================
Does anyone know the problem or meet the problem before?
Thanks a lot!