High GC rich genomic DNA

[Smriti]

Hi Folks,

I need to order some higher GC content genomic DNA. Any suggestions from where to buy?

I found out Streptomyces could be one possibility.

Thanks!

[krobison]

What purpose do you need it for?

Streptomyces are easy to order from ATCC or other culture collections (they're also fun to isolate from soil, but you would need to confirm identity before proceeding). I don't know if you can actually buy the DNA; you will probably need to grow that up yourself.

Micrococcus luteus is a distantly related high G+C bacterium that is often used in undergraduate laboratory exercises. DNA available from Sigma.

If you really want to test a method on high G+C, these are better choices than some of the common used bugs for this such as Bordetella or Rhodobacter, as they are just not as extreme as Streptomyces or Micrococcus.

[Smriti]

Thanks Krobison,

For now I'll order Micrococcus from Sigma. I need to test a high G-C content DNA for library preparation. Hope this one will fulfill it.

Bordetella or Rhodobacter, from where can I buy these? Are these easier to work on than Micrococcus?