Dear All,
As a part of my curriculum, i am currently planning to do a pipeline like sort on NGS data. Through various reading and other sorts i found that there are some leading tools like bowtie, tophat, Maq, Cufflinks etc.
From NCBI SRA sample of RNASEQ of illumina. but as i am new to these technologies i dont know what are the sequential steps i should follow to get a meaning full result.
what i am trying to analyse is to get the differential expression between two different organ data. How can i identify the control sample(is it needed?), and what are the tools i should use and in which order.
I know this will be a mundane task for some of you, but i am hopefull that you people can help me to sort this out.
Hoping for a positive replay asap
As a part of my curriculum, i am currently planning to do a pipeline like sort on NGS data. Through various reading and other sorts i found that there are some leading tools like bowtie, tophat, Maq, Cufflinks etc.
From NCBI SRA sample of RNASEQ of illumina. but as i am new to these technologies i dont know what are the sequential steps i should follow to get a meaning full result.
what i am trying to analyse is to get the differential expression between two different organ data. How can i identify the control sample(is it needed?), and what are the tools i should use and in which order.
I know this will be a mundane task for some of you, but i am hopefull that you people can help me to sort this out.
Hoping for a positive replay asap
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