Seqanswers Leaderboard Ad

Collapse

Announcement

Collapse
No announcement yet.
X
 
  • Filter
  • Time
  • Show
Clear All
new posts

  • samtools c++ api

    I would like to read and write bam files within c++. For that I downloaded the source code files and tried to get the SAMtools Example c program running.

    Upon compiling I get an Error message

    ../src/samtools/bam_reheader.c: In function ‘bam_reheader’:
    ../src/samtools/bam_reheader.c:28: error: ‘BGZF’ has no member named ‘x’

    In "bam_reheader" I found:
    Code:
    // line 10: BGZF *fp;
    #ifdef _USE_KNETFILE
    	while ((len = knet_read(in->x.fpr, buf, BUF_SIZE)) > 0)
    #else
    	while (!feof(in->file) && (len = fread(buf, 1, BUF_SIZE, in->file)) > 0)
    #endif
    		fwrite(buf, 1, len, fp->x.fpw);           // this is line 28
    where line 28 obviously is not affected by the definition of _USE_KNETFILE.

    Upon looking up the definition of BGZF which is located in "bgzf.h", I found the following:
    Code:
    #ifdef _USE_KNETFILE
    	union {
    		knetFile *fpr;
    		FILE *fpw;
    	} x;
    So, when _USE_KNETFILE is not declared, the member x is not included in the declaration of BGZF but expected to exist later on. At first sight, this seems to be an error in the source code.

    Did anyone else encounter this problem or does anyone know a solution?

    Thanks very much.
    Wolfgang

  • #2
    Try posting to the samtools help list: [email protected]

    Comment


    • #3
      Sent a copy

      Originally posted by nilshomer View Post
      Try posting to the samtools help list: [email protected]
      Yes, I Found the SourceForge support Site with your remark.
      Sent a copy of the post.

      Thank you
      Wolfgang

      Comment

      Latest Articles

      Collapse

      • seqadmin
        Current Approaches to Protein Sequencing
        by seqadmin


        Proteins are often described as the workhorses of the cell, and identifying their sequences is key to understanding their role in biological processes and disease. Currently, the most common technique used to determine protein sequences is mass spectrometry. While still a valuable tool, mass spectrometry faces several limitations and requires a highly experienced scientist familiar with the equipment to operate it. Additionally, other proteomic methods, like affinity assays, are constrained...
        04-04-2024, 04:25 PM
      • seqadmin
        Strategies for Sequencing Challenging Samples
        by seqadmin


        Despite advancements in sequencing platforms and related sample preparation technologies, certain sample types continue to present significant challenges that can compromise sequencing results. Pedro Echave, Senior Manager of the Global Business Segment at Revvity, explained that the success of a sequencing experiment ultimately depends on the amount and integrity of the nucleic acid template (RNA or DNA) obtained from a sample. “The better the quality of the nucleic acid isolated...
        03-22-2024, 06:39 AM

      ad_right_rmr

      Collapse

      News

      Collapse

      Topics Statistics Last Post
      Started by seqadmin, 04-11-2024, 12:08 PM
      0 responses
      18 views
      0 likes
      Last Post seqadmin  
      Started by seqadmin, 04-10-2024, 10:19 PM
      0 responses
      22 views
      0 likes
      Last Post seqadmin  
      Started by seqadmin, 04-10-2024, 09:21 AM
      0 responses
      16 views
      0 likes
      Last Post seqadmin  
      Started by seqadmin, 04-04-2024, 09:00 AM
      0 responses
      46 views
      0 likes
      Last Post seqadmin  
      Working...
      X