Hello,
Does anyone use the Covaris to shear their samples in the Roche 3kb paired end library preparation step of circularised DNA fragmentation?
If so, what settings do you use?
I used these Covaris settings:
duty cycle - 5; intensity - 5; cycle/burst - 500; time - 120secs
and got the attached Agilent result.
Mahalo
Does anyone use the Covaris to shear their samples in the Roche 3kb paired end library preparation step of circularised DNA fragmentation?
If so, what settings do you use?
I used these Covaris settings:
duty cycle - 5; intensity - 5; cycle/burst - 500; time - 120secs
and got the attached Agilent result.
Mahalo
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